How to reconstitute 100U Botox bottle

The above video made by me on YouTube explains “how to make Botox“, well, we’re not making the Botox, we are reconstituting it from a dry Botox form to a liquid Botox injectable form.  You could mix it with as much saline as you like.  Some would call this dilution, but I prefer the word reconstitution.  Dilution implies that we are taking a known dose of Botox and making it a lesser dose.

I like 1cc = 4U of Botox

But it works well for me to place 2.5 cc’s of saline into the 100U bottle.  This way every 1.0 cc of saline results in 4 Units of Botox.

Much of how Botox is injected now involves 4U of Botox in each injection site.  Thus this would be 0.1 cc’s of Botox – and conveniently this shows up on the line in our syringes.

Some tips: When inserting a needle into the rubber top of the Botox container, consider using a 21 G needle rather than 18 G needle. Because the 18 G needle might damage the self-sealing aspect of the rubber top.

There are always subtleties and the video might give you more insight into the way I do it, which has worked well for me.

If you are curious, there are some questions in the comment section of the video. Someone asked where I get my medical degree. It was at Brown University.  And someone asked why I wear a mask.  I usually don’t talk when I’m reconstituting Botox, and I consider it to be a medication that I’m making that gets injected into a patient, I wanted to make sure I treat it like a surgery – for much of the same reasons – keep the work area from potential contamination.  Extra talking creates microdroplets from our mouth into the air.  And thus… I prefer to wear a mask if I’m doing some extra talking – for the camera or with a patient while working with Botox.  It may be a surgeon thing.  It makes me more comfortable knowing that I offered the best for my patients.

I hope the info helps.  If you are a potential patient, feel free to visit my page about Botox and we are located in Modesto, California.